Zebra Fish lab located in the Robert Karp Building on the Main Campus of Boston Children's Hospital-Animal Facility. The project consisted of removing 5000 square feet of Zebra Fish holding tanks which housed 250-300 thousand experimental zebra fish. The project was split into 2 phases to accommodate user request with maintaining daily activities on phase 2 with work being completed on phase 1. This phasing resulted in FLC maintaining stringent No-dust/No Vibration requirements so not to interfere with the Zebra Fish breading cycles. FLC utilized multiple negative machines with sealed moister resistant barriers and doors with magnehelic’s to confirm room balance, Work Scope (Phase 1) consisted of demoing 10 rows of holding tanks 8’H x 24”D x 40’L and associated plumbing and power, existing GWB ceiling was removed to accommodate new HVAC Phoenix Valve and S.S. duct work. R.O. water and waste lines were cored down thru the existing floor into the Bertucci's restaurant ACT ceiling area and tied into existing plumbing lines. 14- 12” x 12” Floor sinks were installed for water over- flow mitigation. New 800 AMP 3 panel service was completed to power new holding tanks, pump filtering system, & Auto robot fish feeder system. Multiple plumbing and power shutdowns were coordinated with the in-house engineering so not to interfere with the above animal facility’s daily activities. Final finishes consisted of all moister resistant products (Epoxy Flooring/Phenolic Casework and Sinks/Epoxy Paint). New holding tanks were supplied by Technoplast from Italy with final installation coordinated thru FLC with M.E.P. hookups and monitoring controls. FLC facilitated detailed schedules with 6-week look-a-head’s required by the Animal Facility leadership. Phase 2 was a mirror image in work scope and sequence to phase 1, total project start to completion time was 6 months.